Plasma Lipoproteins Enhance Tissue Factor–Independent Factor VII Activation
نویسندگان
چکیده
منابع مشابه
Plasma lipoproteins enhance tissue factor-independent factor VII activation.
The effect of plasma lipoprotein fractions (large very-low-density lipoprotein, small very-low-density lipoprotein, intermediate-density lipoprotein, and low-density lipoprotein) on initiation of blood coagulation by supporting factor VII activation or by stimulating monocytes to express tissue factor was investigated in vitro. Endotoxin-free preparations of lipoprotein fractions did not induce...
متن کاملPurification of Factor VII from bovine plasma. Reaction with tissue factor and activation of Factor X.
Factor VII has been purified up to 330,000-fold from bovine plasma by adsorption on, and elution from, barium sulfate, anion exchange chromatography, gel filtration, and affinity chromatography on benzamidine coupled to Sepharose. The purified material is inhibited by diisopropylphosphorofluoridate both in its native state and after the formation of a complex with tissue factor in the presence ...
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Although the majority of factor VII (FVII) circulates in the zymogen form, low levels of activated factor VII (FVIIa) have been postulated to exist in plasma and to serve a priming function for triggering of the clotting cascade. However, direct measurement of plasma FVIIa has not previously been possible. We have quantified plasma FVIIa levels using a novel, highly sensitive assay that is free...
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We have used activation peptide release assays to compare factor VII and activated factor VII (VIIa) activation of factor X, normal factor IX (IXN), and a variant factor IX (IXBmLE), which, after activation, is unable to back-activate factor VII. In purified systems, factor VII and VIIa each rapidly activated factor X, but after a one minute lag for factor VII. VIIa also readily activated both ...
متن کاملPurification of Factor VII from Bovine Plasma
Factor VII has been purified up to 330,000-fold from bovine plasma by adsorption on, and elution from, barium sulfate, anion exchange chromatography, gel filtration, and affinity chromatography on benzamidine coupled to Sepharose. The purified material is inhibited by diisopropylphosphorofluoridate both in its native state and after the formation of a complex with tissue factor in the presence ...
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ژورنال
عنوان ژورنال: Arteriosclerosis, Thrombosis, and Vascular Biology
سال: 2000
ISSN: 1079-5642,1524-4636
DOI: 10.1161/01.atv.20.7.1835